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Proceedings Paper

Annular aperture detection scheme in radially polarized coherent anti-Stokes Raman scattering (RP-CARS) microscopy for contrast improvement
Author(s): Jian Lin; Fake Lu; Haifeng Wang; Wei Zheng; C. J. R. Sheppard; Zhiwei Huang
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Paper Abstract

We report on a unique annular aperture detection scheme in radially polarized coherent anti-Stokes Raman scattering (RP-CARS) microscopy to effectively remove the solvent background for high contrast vibrational imaging. Our finite-difference time-domain (FDTD) calculations show that the far-field RP-CARS radiation from the scatterer with size comparable to the excitation wavelength is stronger than that from the solvent at large cone angles (around 45° to 150°). The annular detection provides about one order higher contrast for both forward and backward detected RP-CARS microscopy.

Paper Details

Date Published: 26 February 2010
PDF: 4 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756939 (26 February 2010); doi: 10.1117/12.842942
Show Author Affiliations
Jian Lin, National Univ. of Singapore (Singapore)
Fake Lu, National Univ. of Singapore (Singapore)
Haifeng Wang, Data Storage Institute, A*STAR (Singapore)
Wei Zheng, National Univ. of Singapore (Singapore)
C. J. R. Sheppard, National Univ. of Singapore (Singapore)
Zhiwei Huang, National Univ. of Singapore (Singapore)


Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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