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Proceedings Paper

EGF targeted fluorescence molecular tomography as a predictor of PDT outcomes in pancreas cancer models
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Paper Abstract

Verteporfin photodynamic therapy (PDT) is a promising adjuvant therapy for pancreas cancer and investigations for its use are currently underway in both orthotopic xenograft mouse models and in human clinical trials. The mouse models have been studied extensively using magnetic resonance (MR) imaging as a measure of surrogate response to verteporfin PDT and it was found that tumor lines with different levels of aggression respond with varying levels to PDT. MR imaging was successful in determining the necrotic volume caused by PDT but there was difficultly in distinguishing inflamed tissues and regions of surviving tumor. In order to understand the molecular changes within the tumor immediately post-PDT we propose the implementation of MR-guided fluorescence molecular tomography (FMT) in conjunction with an exogenously administered fluorescently labeled epidermal growth factor (EGF-IRDye800CW, LI-COR Biosciences). We have previously shown that MR-guided FMT is feasible in the mouse abdomen when multiple regions of fluorescence are considered from contributing internal organs. In this case the highly aggressive AsPC-1 (+EGFR) orthotopic tumor was implanted in SCID mice, interstitial verteporfin PDT (1mg/kg, 20J/cm) was performed when the tumor reached ~60mm3 and both tumor volume and EGF binding were followed with MR-guided FMT.

Paper Details

Date Published: 2 March 2010
PDF: 7 pages
Proc. SPIE 7551, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIX, 75510Q (2 March 2010); doi: 10.1117/12.842934
Show Author Affiliations
Kimberley S. Samkoe, Dartmouth College (United States)
Scott C. Davis, Dartmouth College (United States)
Subhadra Srinivasan, Dartmouth College (United States)
Martin E. Isabelle, Dartmouth College (United States)
Julia O'Hara, Dartmouth College (United States)
Tayyaba Hasan, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Brian W. Pogue, Dartmouth College (United States)

Published in SPIE Proceedings Vol. 7551:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIX
David H. Kessel, Editor(s)

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