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Proceedings Paper

Topical photosan-mediated photodynamic therapy for DMBA-induced hamster buccal pouch premaligant lesions: an in vivo study
Author(s): Yih-Chih Hsu; Chun-Pin Chiang; Jian Wen Chen; Ying-Ru Chen; Jeng-Woei Lee
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Paper Abstract

One of the best strategies to prevent the occurrence of oral cancer is to eliminate oral precancers and block their further malignant transformation. Previous studies showed that photosan-mediated photodynamic therapy (photosan-PDT) is very effective for human head and neck cancers. To avoid the systemic photodynamic toxicity of photosan, this study was designed to use a topical photosan-PDT for treatment of DMBA-induced hamster buccal pouch precancerous lesions. Twelve 10-week-old male Syrian golden hamsters were used in this study. DMBA was applied to the left buccal pouches thrice a week for 8 to 10 weeks and mineral oil was painted on the right buccal pouches thrice a week for 8 to 10 weeks as the normal controls. Six hamsters were euthanized for tissue harvest. Precancerous lesions of moderate to severe dysplasia were consistently induced and proven by histological examination. These induced precancerous lesions in the remaining 6 hamsters were used for testing the efficacy of topical photosan-PDT. Before PDT, fluorescence spectroscopy was used to determine when protoporphyrine IX (PpIX) reached its peak level in the lesional epithelial cells after topical application of photosan-gel. We found that PpIX reached its peak level in precancerous lesions about 13.5 min after topical application of photosan-gel. The precancerous lesions in 4 hamsters were treated with topical photosan-PDT using the 635-nm LED light once or twice a week. Complete regression of the precancerous lesions was found after 2-4 PDT treatments by visual and histological examination. Our findings indicate that topical photosan-PDT is a very effective treatment modality for DMBA-induced hamster buccal pouch precancerous lesions.

Paper Details

Date Published: 11 February 2010
PDF: 7 pages
Proc. SPIE 7565, Biophotonics and Immune Responses V, 756509 (11 February 2010); doi: 10.1117/12.842912
Show Author Affiliations
Yih-Chih Hsu, Chung Yuan Christian Univ. (Taiwan)
Chun-Pin Chiang, National Taiwan Univ. (Taiwan)
Jian Wen Chen, Chung Yuan Christian Univ. (Taiwan)
Ying-Ru Chen, Chung Yuan Christian Univ. (Taiwan)
Jeng-Woei Lee, Tzu Chi Univ. (Taiwan)


Published in SPIE Proceedings Vol. 7565:
Biophotonics and Immune Responses V
Wei R. Chen, Editor(s)

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