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Proceedings Paper

Label-free quantitative detection of biomarkers
Author(s): Digant P. Davé; Sajal Chirvi
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Paper Abstract

A highly sensitive technique for detection and quantification of protein-analyte binding interaction using spectral domain low coherence interferometric technique is implemented. Using this technique, it is possible to study real time interactions by quantifying the changes in optical path length caused by protein-analyte binding. The technique does not require elaborate sensor preparation and calibration. Any coverslip with certain thickness and capability to bind protein can work using this technique. For proof of principle, the interaction between IgG antigen and anti-IgG was studied and quantification of the accumulation of anti-IgG on sensor surface was demonstrated. Additionally, for biological relevance interactions between cancer biomarker EGFr and EGF was also studied and the preliminary results are presented. The potential applications of the label free technique include point-of-care screening of cancer biomarkers and tumor cell adhesion analysis.

Paper Details

Date Published: 25 February 2010
PDF: 5 pages
Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 75680Z (25 February 2010); doi: 10.1117/12.842879
Show Author Affiliations
Digant P. Davé, The Univ. of Texas at Arlington (United States)
Sajal Chirvi, The Univ. of Texas at Arlington (United States)

Published in SPIE Proceedings Vol. 7568:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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