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Proceedings Paper

Effects of spatial and spectral frequencies on wide-field functional imaging (wifi) characterization of preclinical breast cancer models
Author(s): Austin Moy; Jae G. Kim; Eva Y. H. P. Lee; Bernard Choi
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Paper Abstract

A common strategy to study breast cancer is the use of the preclinical model. These models provide a physiologically relevant and controlled environment in which to study both response to novel treatments and the biology of the cancer. Preclinical models, including the spontaneous tumor model and mammary window chamber model, are very amenable to optical imaging and to this end, we have developed a wide-field functional imaging (WiFI) instrument that is perfectly suited to studying tumor metabolism in preclinical models. WiFI combines two optical imaging modalities, spatial frequency domain imaging (SFDI) and laser speckle imaging (LSI). Our current WiFI imaging protocol consists of multispectral imaging in the near infrared (650-980 nm) spectrum, over a wide (7 cm x 5 cm) field of view. Using SFDI, the spatially-resolved reflectance of sinusoidal patterns projected onto the tissue is assessed, and optical properties of the tissue are determined, which are then used to extract tissue chromophore concentrations in the form of oxy-, deoxy-, and total hemoglobin concentrations, and percentage of lipid and water. In the current study, we employ Monte Carlo simulations of SFDI light propagation in order to characterize the penetration depth of light in both the spontaneous tumor model and mammary window chamber model. Preliminary results suggest that different spatial frequency and wavelength combinations have different penetration depths, suggesting the potential depth sectioning capability of the SFDI component of WiFI.

Paper Details

Date Published: 26 February 2010
PDF: 6 pages
Proc. SPIE 7573, Biomedical Applications of Light Scattering IV, 757309 (26 February 2010); doi: 10.1117/12.842829
Show Author Affiliations
Austin Moy, Beckman Laser Institute and Medical Ctr. (United States)
Univ. of California, Irvine (United States)
Jae G. Kim, Beckman Laser Institute and Medical Ctr. (United States)
Eva Y. H. P. Lee, Univ. of California, Irvine (United States)
Bernard Choi, Beckman Laser Institute and Medical Ctr. (United States)
Univ. of California, Irvine (United States)


Published in SPIE Proceedings Vol. 7573:
Biomedical Applications of Light Scattering IV
Adam P. Wax; Vadim Backman, Editor(s)

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