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Proceedings Paper

Monitor hemoglobin concentration and oxygen saturation in living mouse tail using photoacoustic CT scanner
Author(s): Bo Liu; Robert Kruger; Daniel Reinecke; Keith M. Stantz
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Paper Abstract

Purpose: The purpose of this study is to use PCT spectroscopy scanner to monitor the hemoglobin concentration and oxygen saturation change of living mouse by imaging the artery and veins in a mouse tail. Materials and Methods: One mouse tail was scanned using the PCT small animal scanner at the isosbestic wavelength (796nm) to obtain its hemoglobin concentration. Immediately after the scan, the mouse was euthanized and its blood was extracted from the heart. The true hemoglobin concentration was measured using a co-oximeter. Reconstruction correction algorithm to compensate the acoustic signal loss due to the existence of bone structure in the mouse tail was developed. After the correction, the hemoglobin concentration was calculated from the PCT images and compared with co-oximeter result. Next, one mouse were immobilized in the PCT scanner. Gas with different concentrations of oxygen was given to mouse to change the oxygen saturation. PCT tail vessel spectroscopy scans were performed 15 minutes after the introduction of gas. The oxygen saturation values were then calculated to monitor the oxygen saturation change of mouse. Results: The systematic error for hemoglobin concentration measurement was less than 5% based on preliminary analysis. Same correction technique was used for oxygen saturation calculation. After correction, the oxygen saturation level change matches the oxygen volume ratio change of the introduced gas. Conclusion: This living mouse tail experiment has shown that NIR PCT-spectroscopy can be used to monitor the oxygen saturation status in living small animals.

Paper Details

Date Published: 24 February 2010
PDF: 5 pages
Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 756439 (24 February 2010); doi: 10.1117/12.842827
Show Author Affiliations
Bo Liu, Purdue Univ. (United States)
Robert Kruger, OptoSonics, Inc. (United States)
Daniel Reinecke, OptoSonics, Inc. (United States)
Keith M. Stantz, Purdue Univ. (United States)
Indiana Univ. (United States)


Published in SPIE Proceedings Vol. 7564:
Photons Plus Ultrasound: Imaging and Sensing 2010
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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