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Proceedings Paper

Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells
Author(s): YongKeun Park; Toyohiko Yamauchi; Wonshik Choi; Ramachandra Dasari; Michael S. Feld
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Paper Abstract

We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sampleinduced phase shifts (for the detail, please see the Ref. [1]). Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual red blood cells (RBCs). The end point of this study is non-invasive monitoring of physiological states of intact living cells.

Paper Details

Date Published: 25 February 2010
PDF: 5 pages
Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 756812 (25 February 2010); doi: 10.1117/12.842813
Show Author Affiliations
YongKeun Park, Massachusetts Institute of Technology (United States)
Toyohiko Yamauchi, Massachusetts Institute of Technology (United States)
Hamamatsu Photonics K. K. (Japan)
Wonshik Choi, Massachusetts Institute of Technology (United States)
Korea Univ. (Korea, Republic of)
Ramachandra Dasari, Massachusetts Institute of Technology (United States)
Michael S. Feld, Massachusetts Institute of Technology (United States)


Published in SPIE Proceedings Vol. 7568:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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