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Proceedings Paper

Speckle-field digital holographic microscopy
Author(s): YongKeun Park; Wonshik Choi; Zahid Yaqoob; Ramachandra Dasari; Kamran Badizadegan; Michael S. Feld
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Paper Abstract

We report a technique which can overcome these drawbacks, but maintains the advantage of phase microscopy - high contrast live cell imaging and 3D imaging (For the detail, please see the Ref. [1]). A speckle beam of a complex spatial pattern is used for illumination to reduce fixed pattern noise and to improve optical sectioning capability. By recording of the electric field of speckle, we demonstrate high contrast 3D live cell imaging without the need for axial scanning - neither objective lens nor sample stage. This technique has great potential in studying biological samples with improved sensitivity, resolution and optical sectioning capability.

Paper Details

Date Published: 24 February 2010
PDF: 5 pages
Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 75681O (24 February 2010); doi: 10.1117/12.842799
Show Author Affiliations
YongKeun Park, Massachusetts Institute of Technology (United States)
Wonshik Choi, Massachusetts Institute of Technology (United States)
Korea Univ. (Korea, Republic of)
Zahid Yaqoob, Massachusetts Institute of Technology (United States)
Ramachandra Dasari, Massachusetts Institute of Technology (United States)
Kamran Badizadegan, Massachusetts Institute of Technology (United States)
Harvard Medical School (United States)
Massachusetts General Hospital (United States)
Michael S. Feld, Massachusetts Institute of Technology (United States)


Published in SPIE Proceedings Vol. 7568:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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