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Proceedings Paper

Near-field optical fluorescence correlation spectroscopy
Author(s): José Pérez; Michael Herrmann; Dietmar Gradl; Andreas Naber
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Paper Abstract

The application of Fluorescence Correlation Spectroscopy (FCS) to Near-field Scanning Optical Microscopy on diffusing molecules in solution is presented here. The ultra small excitation volume in such a technique allows measurements with fluorophore concentrations of up to 10μM, making FCS in biological environment at nearly native conditions possible.

Paper Details

Date Published: 26 February 2010
PDF: 9 pages
Proc. SPIE 7571, Single Molecule Spectroscopy and Imaging III, 757118 (26 February 2010); doi: 10.1117/12.842793
Show Author Affiliations
José Pérez, Karlsruhe Institute of Technology (Germany)
Michael Herrmann, Karlsruhe Institute of Technology (Germany)
Dietmar Gradl, Karlsruhe Institute of Technology (Germany)
Andreas Naber, Karlsruhe Institute of Technology (Germany)


Published in SPIE Proceedings Vol. 7571:
Single Molecule Spectroscopy and Imaging III
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann, Editor(s)

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