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Proceedings Paper

Dual-mode digital holographic and fluorescence microscopy for the study of morphological changes in cells under simulated microgravity
Author(s): M. Fatih Toy; Christophe Pache; Jérôme Parent; Jonas Kühn; Marcel Egli; Christian Depeursinge
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Paper Abstract

A dual mode microscope is developed to study morphological evolution of mouse myoblast cells under simulated microgravity in real time. Microscope operates in Digital Holographic Microscopy (DHM) and widefield epifluorescence microscopy modes in a time sequential basis. DHM provides information on real time cellular morphology. EGFP transfected actin filaments in mouse myoblast cells function as the reporter for the fluorescence microscopy mode. Experimental setup is fixed in the RPM to observe microgravity induced dynamic changes in live cells. Initial results revealed two different modifications. Disorganized structures become visible in the formed lamellipodias, and proteins accumulate in the perinuclear region.

Paper Details

Date Published: 24 February 2010
PDF: 4 pages
Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 75700A (24 February 2010); doi: 10.1117/12.842779
Show Author Affiliations
M. Fatih Toy, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Christophe Pache, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Jérôme Parent, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Jonas Kühn, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Marcel Egli, ETH Zurich (Switzerland)
Christian Depeursinge, Ecole Polytechnique Fédérale de Lausanne (Switzerland)


Published in SPIE Proceedings Vol. 7570:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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