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Proceedings Paper

Fluorescence correlation spectroscopy on nano-fakir surfaces
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Paper Abstract

Single biomolecule behaviour can reveal crucial information about processes not accessible by ensemble measurements. It thus represents a real biotechnological challenge. Common optical microscopy approaches require pico- to nano-molar concentrations in order to isolate an individual molecule in the observation volume. However, biologically relevant conditions often involve micromolar concentrations, which impose a drastic reduction of the conventional observation volume by at least three orders of magnitude. This confinement is also crucial for mapping sub-wavelength heterogeneities in cells, which play an important role in many biological processes. We propose an original approach, which couples Fluorescence Correlation Spectroscopy (FCS), a powerful tool to retrieve essential information on single molecular behaviour, and nano-fakir substrates with strong field enhancements and confinements at their surface. These electromagnetic singularities at nanometer scale, called "hotspots", are the result of the unique optical properties of surface plasmons. They provide an elegant means for studying single-molecule dynamics at high concentrations by reducing dramatically the excitation volume and enhancing the fluorophore signal by several orders of magnitude. The nano-fakir substrates used are obtained from etching optical fiber bundles followed by sputtering of a gold thin-film. It allows one to design reproducible arrays of nanotips.

Paper Details

Date Published: 25 February 2010
PDF: 7 pages
Proc. SPIE 7571, Single Molecule Spectroscopy and Imaging III, 757114 (25 February 2010); doi: 10.1117/12.842763
Show Author Affiliations
Julie Delahaye, Univ. Denis Diderot, Institut Langevin, CNRS (France)
Samuel Gresillon, Univ. Pierre et Marie Curie, Institut Langevin, CNRS (France)
Sandrine Lévêque-Fort, Lab. de Photophysique Moléculaire CNRS, Univ. Paris-Sud 11 (France)
Neso Sojic, Institut des Sciences Moléculaires, Univ. Bordeaux 1, CNRS (France)
Emmanuel Fort, Univ. Denis Diderot, Institut Langevin, CNRS (France)

Published in SPIE Proceedings Vol. 7571:
Single Molecule Spectroscopy and Imaging III
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann, Editor(s)

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