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Proceedings Paper

Transferrin protein nanospheres: a nanoplatform for receptor-mediated cancer cell labeling and gene delivery
Author(s): Michael A. McDonald; Tighe A. Spurlin; Alessandro Tona; John T. Elliott; Michael Halter; Anne L. Plant
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Paper Abstract

This paper presents preliminary results on the use of transferrin protein nanospheres (TfpNS) for targeting cancer cells in vitro. Protein nanospheres represent an easily prepared and modifiable nanoplatform for receptor-specific targeting, molecular imaging and gene delivery. Rhodamine B isothiocyanate conjugated TfpNS (RBITC-TfpNS) show significantly enhanced uptake in vitro in SK-MEL-28 human malignant melanoma cells known to overexpress transferrin receptors compared to controls. RBITCTfpNS labeling of the cancer cells is due to transferrin receptor-mediated uptake, as demonstrated by competitive inhibition with native transferrin. Initial fluorescence microscopy studies indicate GFP plasmid can be transfected into melanoma cells via GFP plasmid encapsulated by TfpNS.

Paper Details

Date Published: 17 February 2010
PDF: 12 pages
Proc. SPIE 7576, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II, 75760J (17 February 2010); doi: 10.1117/12.842757
Show Author Affiliations
Michael A. McDonald, Chemical Science and Technology Lab., NIST (United States)
Tighe A. Spurlin, Chemical Science and Technology Lab., NIST (United States)
Alessandro Tona, Chemical Science and Technology Lab., NIST (United States)
Science Applications International Corp. (United States)
John T. Elliott, Chemical Science and Technology Lab., NIST (United States)
Michael Halter, Chemical Science and Technology Lab., NIST (United States)
Anne L. Plant, Chemical Science and Technology Lab., NIST (United States)


Published in SPIE Proceedings Vol. 7576:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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