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Proceedings Paper

Multimodal confocal mosaicing microscopy: an emphasis on squamous cell carcinoma
Author(s): Nathaniel W. Chen; Jordan Sensibaugh; Ardaland Ardeshiri; Adam Blanchard; Steven Jacques; Daniel Gareau
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Paper Abstract

Our previous study reported a sensitivity of 96.6% and a specificity of 89.2% in rapidly detecting Basal Cell Carcinomas (BCCs) when nuclei were stained with acridine orange. Squamous Cell Carcinomas (SCCs) and infiltrative BCCs remain difficult to detect. More complete screening can be achieved utilizing both acridine orange for nuclei staining and eosin for cytoplasmic contrast, using two lasers to excite the two stains independently. Nuclear fluorescence is achieved by staining with acridine orange (0.5mM, 60 s), and cytoplasmic fluorescence is achieved by staining with eosin working solution (30 s). This work shows good morphological contrast of SCC and infiltrative BCC with eosin, acridine orange, and reflectance, and presents a means for rapid SCC and infiltrative BCC detection in fresh skin excisions using multimodal confocal microscopy. In addition, digital staining is shown to effectively simulate hematoxylin and eosin (H&E) histology with confocal mosaics.

Paper Details

Date Published: 2 March 2010
PDF: 7 pages
Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75480K (2 March 2010); doi: 10.1117/12.842746
Show Author Affiliations
Nathaniel W. Chen, Oregon Health & Science Univ. (United States)
Jordan Sensibaugh, Oregon Health & Science Univ. (United States)
Ardaland Ardeshiri, Oregon Health & Science Univ. (United States)
Adam Blanchard, Oregon Health & Science Univ. (United States)
Steven Jacques, Oregon Health & Science Univ. (United States)
Daniel Gareau, Oregon Health & Science Univ. (United States)

Published in SPIE Proceedings Vol. 7548:
Photonic Therapeutics and Diagnostics VI
Anita Mahadevan-Jansen; Andreas Mandelis; Brian Jet-Fei Wong M.D.; Nikiforos Kollias; Henry Hirschberg M.D.; Kenton W. Gregory M.D.; Reza S. Malek; E. Duco Jansen; Guillermo J. Tearney; Steen J. Madsen; Bernard Choi; Justus F. R. Ilgner; Haishan Zeng; Laura Marcu, Editor(s)

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