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Proceedings Paper

Tumor specific lung cancer diagnostics with multiplexed FRET immunoassays
Author(s): D. Geißler; D. Hill; H.-G. Löhmannsröben; E. Thomas; A. Lavigne; B. Darbouret; E. Bois; L. J. Charbonnière; R. F. Ziessel; N. Hildebrandt
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Paper Abstract

An optical multiplexed homogeneous (liquid phase) immunoassay based on FRET from a terbium complex to eight different fluorescent dyes is presented. We achieved highly sensitive parallel detection of four different lung cancer specific tumor markers (CEA, NSE, SCC and CYFRA21-1) within a single assay and show a proof-of-principle for 5- fold multiplexing. The method is well suited for fast and low-cost miniaturized point-of-care testing as well as for highthroughput screening in a broad range of in-vitro diagnostic applications.

Paper Details

Date Published: 26 February 2010
PDF: 6 pages
Proc. SPIE 7572, Optical Diagnostics and Sensing X: Toward Point-of-Care Diagnostics, 75720C (26 February 2010); doi: 10.1117/12.842731
Show Author Affiliations
D. Geißler, Univ. Potsdam (Germany)
D. Hill, Univ. Potsdam (Germany)
H.-G. Löhmannsröben, Univ. Potsdam (Germany)
E. Thomas, Cezanne S.A.S. (France)
A. Lavigne, Cezanne S.A.S. (France)
B. Darbouret, Cezanne S.A.S. (France)
E. Bois, Cezanne S.A.S. (France)
L. J. Charbonnière, Lab. d'Ingénierie Moléculaire Analytique, CNRS, Univ. Louis Pasteur (France)
R. F. Ziessel, Lab. de Chimie Organique et Spectroscopie Avancée, CNRS, Univ. Louis Pasteur (France)
N. Hildebrandt, Fraunhofer-Institut für Angewandte Polymerforschung (Germany)

Published in SPIE Proceedings Vol. 7572:
Optical Diagnostics and Sensing X: Toward Point-of-Care Diagnostics
Gerard L. Coté, Editor(s)

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