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Proceedings Paper

Evaluation of Her2 status using photoacoustic spectroscopic CT techniques
Author(s): Michael Shaffer; Robert Kruger; Daniel Reinecke; Helen Chin-Sinex; Marc Mendonca; Keith M. Stantz
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Paper Abstract

Purpose: The purpose of this study is to determine the feasibility of using photacoustic CT spectroscopy(PCT-s) to track a near infrared dye conjugated with trastuzumab in vivo. Materials and Methods: An animal model was developed which contained both high and low Her2 expression tumor xenografts on the same mouse. The tumors were imaged at multiple wavelengths (680- 950nm) in the PCT scanner one day prior to injection of the near infrared conjugated probe. Baseline optical imaging data was acquired and the probe was then injected via the tail vein. Fluorescence data was acquired over the next week, PCT spectroscopic data was also acquired during this timeframe. The mice were sacrificed and tumors were extirpated and sent to pathology for IHC staining to verify Her2 expression levels. The optical fluorescence images were analyzed to determine probe uptake dynamics. Reconstructed PCT spectroscopic data was analyzed using IDL routines to deconvolve the probe signal from endogenous background signals, and to determine oxygen saturation. Results: The location of the NIR conjugate was able to be identified within the tumor utilizing IDL fitting routines, in addition oxygen saturation, and hemoglobin concentrations were discernible from the spectroscopic data. Conclusion: Photacoustic spectroscopy allows for the determination of in vivo tumor drug delivery at greater depths than can be determined from optical imaging techniques.

Paper Details

Date Published: 23 February 2010
PDF: 5 pages
Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 756435 (23 February 2010); doi: 10.1117/12.842639
Show Author Affiliations
Michael Shaffer, Purdue Univ. (United States)
Robert Kruger, OptoSonics, Inc. (United States)
Daniel Reinecke, OptoSonics, Inc. (United States)
Helen Chin-Sinex, Indiana Univ. (United States)
Marc Mendonca, Indiana Univ. (United States)
Keith M. Stantz, Purdue Univ. (United States)
Indiana Univ. (United States)

Published in SPIE Proceedings Vol. 7564:
Photons Plus Ultrasound: Imaging and Sensing 2010
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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