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Proceedings Paper

Two-photon microscopy with dynamic focusing objective using a liquid lens
Author(s): Kye-Sung Lee; Philip Vanderwall; Jannick P. Rolland
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Paper Abstract

A variable focus microscope without moving parts for axial focusing within the sample in two-photon microscopy can provide a faster, more robust, and cost-effective solution to 3D imaging at high resolution. A custom dynamic focusing objective was recently developed that incorporates a liquid lens within the optical design of a custom microscope. In this paper, we investigate the use of a liquid lens embedded in an aberration-corrected custom microscope for z-dimension scanning in 3D resolved two-photon microscopy. Results show that we can achieve imaging speed of 50 frames per sec for an arbitrary plane in 3D space, a leap forward in scan speed for two-photon microscopy.

Paper Details

Date Published: 26 February 2010
PDF: 7 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756923 (26 February 2010); doi: 10.1117/12.842581
Show Author Affiliations
Kye-Sung Lee, The Institute of Optics, Univ. of Rochester (United States)
Philip Vanderwall, The Institute of Optics, Univ. of Rochester (United States)
Jannick P. Rolland, The Institute of Optics, Univ. of Rochester (United States)


Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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