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Proceedings Paper

Multimodal nonlinear microscopy at 1.5 µm
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Paper Abstract

We describe multimodal nonlinear microscopy using a compact, turnkey femtosecond fiber laser at 1.5 μm. The system allows for multiplexed detection of near infrared and visible contrast agents through two-and three-photon excitation fluorescence microscopy as well as structural imaging viaauto-confocal microscopy (ACM). This platform expands the available emission spectrum for multiphoton microscopy, enables simultaneous structural and functional imaging, and offers advantages in penetration depth, contrast, and simplicity as compared to conventional MPM near 800 nm excitation.

Paper Details

Date Published: 17 March 2010
PDF: 7 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75691Z (17 March 2010); doi: 10.1117/12.842573
Show Author Affiliations
Chun Zhan, GE Global Research (United States)
Chulmin Joo, GE Global Research (United States)
Qing Li, GE Global Research (United States)
Mikhail Y. Berezin, Washington Univ. School of Medicine (United States)
Walter J. Akers, Washington Univ. School of Medicine (United States)
Yunpeng Ye, Washington Univ. School of Medicine (United States)
Samuel Achilefu, Washington Univ. School of Medicine (United States)
Siavash Yazdanfar, GE Global Research (United States)


Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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