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Proceedings Paper

Real-time megapixel multispectral bioimaging
Author(s): Jason M. Eichenholz; Nick Barnett; Yishung Juang; Dave Fish; Steve Spano; Erik Lindsley; Daniel L. Farkas
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Paper Abstract

Spectral imaging involves capturing images at multiple wavelengths resulting in a data cube (x, y, λ) that allows materials to be identified by its spectral signature. While hyperspectral imagers can provide high spectral resolution, they also have major drawbacks such as cost, size, and the copious amounts of data in the image cube. Typically, the complete hyperspectral data cube provides little additional information compared to only 3-8 discrete (multiwavelength) imaging bands. We present two new approaches and related technologies where we are able to acquire spectral imaging data stacks quickly and cost-effectively. Our two spectral imaging systems represent different approaches integrated with standard CCD and CMOS imagers: sequential rotating filter wheels (RFWs) and lithographically patterned dichroic filter arrays (DFAs). The RFW approach offers the ability for rapid configuration of a spectral system, and a whole new level of self-contained image acquisition, processing and on-board display. The DFA approach offers the potential for ultra compact imagers with acquisition of images of multiple wavelengths simultaneously, while still allowing for processing and display steps to be built into the camera. Both approaches lend themselves production of multi-wavelength/spectral imaging systems with differing features and advantages.

Paper Details

Date Published: 25 February 2010
PDF: 10 pages
Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 75681L (25 February 2010); doi: 10.1117/12.842563
Show Author Affiliations
Jason M. Eichenholz, Ocean Optics, Inc. (United States)
Ocean Thin Films Inc. (United States)
Nick Barnett, Ocean Optics, Inc. (United States)
Yishung Juang, Ocean Optics, Inc. (United States)
Dave Fish, Ocean Thin Films Inc. (United States)
Steve Spano, Finger Lakes Engineering (United States)
Erik Lindsley, Cedars-Sinai Medical Ctr. (United States)
Daniel L. Farkas, Cedars-Sinai Medical Ctr. (United States)


Published in SPIE Proceedings Vol. 7568:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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