Share Email Print
cover

Proceedings Paper

Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi
Author(s): C. V. Stahl; D. B. Almeida; A. A. de Thomaz; A. Fontes; R. F. S. Menna-Barreto; J. R. Santos-Mallet; C. L. Cesar; S. A. O. Gomes; D. Feder
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Many studies have been done in order to verify the possible nanotoxicity of quantum dots in some cellular types. Protozoan pathogens as Trypanosoma cruzi, etiologic agent of Chagas1 disease is transmitted to humans either by blood-sucking triatomine vectors, blood transfusion, organs transplantation or congenital transmission. The study of the life cycle, biochemical, genetics, morphology and others aspects of the T. cruzi is very important to better understand the interactions with its hosts and the disease evolution on humans. Quantum dot, nanocrystals, highly luminescent has been used as tool for experiments in in vitro and in vivo T. cruzi life cycle development in real time. We are now investigating the quantum dots toxicity on T. cruzi parasite cells using analytical methods. In vitro experiments were been done in order to test the interference of this nanoparticle on parasite development, morphology and viability (live-death). Ours previous results demonstrated that 72 hours after parasite incubation with 200 μM of CdTe altered the development of T. cruzi and induced cell death by necrosis in a rate of 34%. QDs labeling did not effect: (i) on parasite integrity, at least until 7 days; (ii) parasite cell dividing and (iii) parasite motility at a concentration of 2 μM CdTe. This fact confirms the low level of cytotoxicity of these QDs on this parasite cell. In summary our results is showing T. cruzi QDs labeling could be used for in vivo cellular studies in Chagas disease.

Paper Details

Date Published: 18 February 2010
PDF: 6 pages
Proc. SPIE 7575, Colloidal Quantum Dots for Biomedical Applications V, 757513 (18 February 2010); doi: 10.1117/12.842558
Show Author Affiliations
C. V. Stahl, Lab. de Transmissores de Leishmanioses, IOC-FIOCRUZ (Brazil)
D. B. Almeida, Lab. de Aplicações Biomédicas de Lasers, Instituto de Física, UNICAMP (Brazil)
A. A. de Thomaz, Lab. de Aplicações Biomédicas de Lasers, Instituto de Física, UNICAMP (Brazil)
A. Fontes, Univ. Federal de Pernambuco (Brazil)
R. F. S. Menna-Barreto, Lab. de Biologia Celular, IOC-FIOCRUZ (Brazil)
J. R. Santos-Mallet, Lab. de Transmissores de Leishmanioses, IOC-FIOCRUZ (Brazil)
C. L. Cesar, Lab. de Aplicações Biomédicas de Lasers, Instituto de Física, UNICAMP (Brazil)
S. A. O. Gomes, Lab. de Transmissores de Leishmanioses, IOC-FIOCRUZ (Brazil)
D. Feder, Lab. de Biologia de Insetos, Univ. Federal Fluminense (Brazil)


Published in SPIE Proceedings Vol. 7575:
Colloidal Quantum Dots for Biomedical Applications V
Marek Osinski; Wolfgang J. Parak; Thomas M. Jovin; Kenji Yamamoto, Editor(s)

© SPIE. Terms of Use
Back to Top