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Proceedings Paper

Rapid line-scan confocal imaging of retinal activation
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Paper Abstract

A rapid line-scan confocal imager was developed for reflected light measurement of fast IOSs correlated with retinal activation. This functional imager provides two, i.e., frame-by-frame and line-by-line, imaging modalities. While frame-by-frame imaging allows dynamic visualization of IOSs over a two-dimensional retinal area at a frame speed > 100 Hz; line-by-line recording can provide ultrafast (> 10 KHz) monitoring of a fixed line area of the retina. A series of experiments was conducted to characterize reflected IOSs in frog retinas, and simultaneous electrophysiological responses were measured. Our experiments indicated that reflected IOSs were tightly correlated with retinal stimuli. Because of effective rejection of out-of-focus background light, rapid confocal imaging typically disclosed fast IOSs with magnitude peak > 30% ▵I/I, where ▵I was dynamic optical change and I was background light intensity. We anticipate that further development of the IOS imaging technology will pave the way toward noninvasive, high resolution evaluation of retinal function.

Paper Details

Date Published: 2 March 2010
PDF: 5 pages
Proc. SPIE 7550, Ophthalmic Technologies XX, 75500D (2 March 2010); doi: 10.1117/12.842526
Show Author Affiliations
Yang-Guo Li, The Univ. of Alabama at Birmingham (United States)
Xin-Cheng Yao, The Univ. of Alabama at Birmingham (United States)

Published in SPIE Proceedings Vol. 7550:
Ophthalmic Technologies XX
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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