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Proceedings Paper

Fs-laser cell perforation using gold nanoparticles of different shapes
Author(s): Markus Schomaker; Holger Fehlauer; Willem Bintig; Anaclet Ngezahayo; Ingo Nolte; Hugo Murua Escobar; Holger Lubatschowski; Alexander Heisterkamp
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Paper Abstract

The resulting effects of the interaction between nanoparticles and laser irradiation are a current matter in research. Depending on the laser parameters as well as the particles properties several effects may occur e.g. bubble formation, melting, fragmentation or an optical breakdown at the surface of the nanoparticle. Besides the investigations of these effects, we employed them to perforate the membrane of different cell lines and investigated nanoparticle mediated laser cell perforation as an alternative optical transfection method. Therefore, the gold nanoparticles (GNP) of different shapes were applied. Furthermore, we varied the methods for attaching GNP to the membrane, i.e. co-incubation of pure gold nanoparticles and bioconjugation of the surface of GNP. The optimal incubation time and the location of the GNP at the cell membrane were evaluated by multiphoton microscopy. If these GNP loaded cells are irradiated with a fs laser beam, small areas of the membrane can be perforated. Following, extra cellular molecules such as membrane impermeable dyes or foreign DNA (GFP vectors) are able to diffuse through the perforated area into the treated cells. We studied the dependence of the laser fluence, GNP concentration, GNP size and shape for successful nanoparticle mediated laser cell perforation. Due to a weak focusing of the laser beam a gentle cell treatment with high cell viabilities and high perforation efficiencies can be achieved. A further advantage of this perforation technique is the high number of cells that can be treated simultaneously. Additionally, we show applications of this method to primary and stem cells.

Paper Details

Date Published: 25 February 2010
PDF: 5 pages
Proc. SPIE 7589, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications X, 75890C (25 February 2010); doi: 10.1117/12.842446
Show Author Affiliations
Markus Schomaker, Laser Zentrum Hannover e.V. (Germany)
Research Cluster of Excellence REBIRTH (Germany)
Holger Fehlauer, Laser Zentrum Hannover e.V. (Germany)
Research Cluster of Excellence REBIRTH (Germany)
Willem Bintig, Leibniz Univ. Hannover (Germany)
Anaclet Ngezahayo, Leibniz Univ. Hannover (Germany)
Ingo Nolte, Research Cluster of Excellence REBIRTH, Univ. of Veterinary Medicine Hannover (Germany)
Hugo Murua Escobar, Research Cluster of Excellence REBIRTH, Univ. of Veterinary Medicine Hannover (Germany)
Holger Lubatschowski, Laser Zentrum Hannover e.V. (Germany)
Research Cluster of Excellence REBIRTH (Germany)
Alexander Heisterkamp, Laser Zentrum Hannover e.V. (Germany)
Research Cluster of Excellence REBIRTH (Germany)


Published in SPIE Proceedings Vol. 7589:
Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications X
Alexander Heisterkamp; Joseph Neev; Stefan Nolte; Rick P. Trebino, Editor(s)

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