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Proceedings Paper

Dynamic contrast enhanced-magnetic resonance imaging (DCE-MRI) for the assessment of Pc 4-sensitized photodynamic therapy of a U87-derived glioma model in the athymic nude rat
Author(s): Ali Anka; Paul Thompson; Eric Mott; Rahul Sharma; Ruozhen Zhang; Nathan Cross; Jiayang Sun; Chris A. Flask; Nancy L. Oleinick; David Dean
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Paper Abstract

Introduction: Dynamic Contrast-Enhanced-Magnetic Resonance Imaging (DCE-MRI) may provide a means of tracking the outcome of Pc 4-sensitized photodynamic therapy (PDT) in deeply placed lesions (e.g., brain tumors). We previously determined that 150 μL of gadolinium (Gd-DTPA) produces optimal enhancement of U87-derived intracerebral tumors in an athymic nude rat glioma model. We wish to determine how consistently DCE-MRI enhancement will detect an increase in Gd-enhancement of these tumors following Pc 4-PDT. Methods: We injected 2.5 x 105 U87 cells into the brains of 6 athymic nude rats. After 7-8 days pre-Pc 4 PDT peri-tumor DCE-MRI images were acquired on a 7.0T microMRI scanner before and after administration of 150 μL Gd. DCE-MRI scans were repeated on Days 11, 12, and 13 following Pc 4-PDT (Day 8 or 9). Results: Useful DCE-MRI data were obtained for these animals before and after Pc 4- PDT. In the pre-Pc 4-PDT DCE-MRI scans an average normalized peak Gd enhancement was observed in tumor tissue that was 1.297 times greater than baseline (0.035 Standard Error [SE]). The average normalized peak Gd enhancement in the tumor tissue in the scan following PDT (Day 11) was 1.537 times greater than baseline (0.036 SE), a statistically significant increase in enhancement (p = 0.00584) over the pre-PDT level. Discussion: A 150 μL Gd dose appears to provide an unambiguous increase in signal indicating Pc 4-PDT-induced necrosis of the U87-derived tumor. Our DCEMRI protocol may allow the development of a clinically robust, unambiguous, non-invasive technique for the assessment of PDT outcome.

Paper Details

Date Published: 3 March 2010
PDF: 8 pages
Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75483X (3 March 2010); doi: 10.1117/12.842232
Show Author Affiliations
Ali Anka, Case Western Reserve Univ. (United States)
Paul Thompson, Case Western Reserve Univ. (United States)
Eric Mott, Case Western Reserve Univ. (United States)
Rahul Sharma, Case Western Reserve Univ. (United States)
Ruozhen Zhang, Case Western Reserve Univ. (United States)
Nathan Cross, Case Western Reserve Univ. (United States)
Jiayang Sun, Case Western Reserve Univ. (United States)
Chris A. Flask, Case Western Reserve Univ. (United States)
Case Comprehensive Cancer Ctr., Case Western Reserve Univ. (United States)
Nancy L. Oleinick, Case Western Reserve Univ. (United States)
Case Comprehensive Cancer Ctr., Case Western Reserve Univ. (United States)
David Dean, Case Western Reserve Univ. (United States)
Case Comprehensive Cancer Ctr., Case Western Reserve Univ. (United States)


Published in SPIE Proceedings Vol. 7548:
Photonic Therapeutics and Diagnostics VI
Anita Mahadevan-Jansen; Andreas Mandelis; Brian Jet-Fei Wong; Nikiforos Kollias; Henry Hirschberg; Kenton W. Gregory; Reza S. Malek; E. Duco Jansen; Guillermo J. Tearney; Steen J. Madsen; Bernard Choi; Justus F. R. Ilgner; Haishan Zeng; Laura Marcu, Editor(s)

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