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Proceedings Paper

Characterizing polarized autofluorescence of normal and benign tissues using singular value decomposition and wavelet transform
Author(s): Anita H. Gharekhan; Siddharth Arora; Ashok N. Oza; M. B. Sureshkumar; Asima Pradhan; Prasanta K. Panigrahi
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Paper Abstract

A systematic investigation of the polarization characteristics of the auto-fluorescence of normal and benign human breast tissues is carried out complementing our earlier studies on normal and cancer tissues. Co- and cross-polarized auto-fluorescence are collected in the 500 to 700nm range through excitation at 488nm using laser as excitation source. A number of parameters, capturing spectral variations are extracted in the co- and cross-polarized channels through singular value decomposition and wavelet decomposition, which differentiate normal and benign tissues. The correlation matrix differs significantly in normal and benign tissues reflecting the presence of different fluorophores. The eigenvectors corresponding to the dominant eigenvalues reveal differences between tissue types. The co-polarized component being sensitive to intrinsic fluorescence shows different behavior for normal and benign tissues in the emission domain of known fluorophores. Interestingly, the benign tissue samples show correlation properties intermediate to malignant and normal cases. In the wavelet domain the standard deviation of percentage fluctuation reveal differences between tissues type. The correlation characteristics manifest prominently in the wavelet low pass (average) domain.

Paper Details

Date Published: 23 February 2010
PDF: 5 pages
Proc. SPIE 7563, Dynamics and Fluctuations in Biomedical Photonics VII, 756308 (23 February 2010); doi: 10.1117/12.842147
Show Author Affiliations
Anita H. Gharekhan, Gujarat Univ. (India)
Siddharth Arora, Univ. of Oxford (United Kingdom)
Ashok N. Oza, Gujarat Univ. (India)
M. B. Sureshkumar, The M. S. Univ. of Baroda (India)
Asima Pradhan, Indian Institute of Technology Kanpur (India)
Prasanta K. Panigrahi, Physical Research Lab. (India)
Indian Institute of Science Education and Research (India)

Published in SPIE Proceedings Vol. 7563:
Dynamics and Fluctuations in Biomedical Photonics VII
Valery V. Tuchin; Donald D. Duncan; Kirill V. Larin, Editor(s)

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