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Proceedings Paper

Dual-beam optical trapping of cells in an optofluidic device fabricated by femtosecond lasers
Author(s): N. Bellini; F. Bragheri; K. C. Vishnubhatla; L. Ferrara; P. Minzioni; G. Cerullo; R. Ramponi; I. Cristiani; R. Osellame
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Paper Abstract

We present design and optimization of an optofluidic monolithic chip, able to provide optical trapping and controlled stretching of single cells. The chip is fabricated in a fused silica glass substrate by femtosecond laser micromachining, which can produce both optical waveguides and microfluidic channels with great accuracy. Versatility and three-dimensional capabilities of this fabrication technology provide the possibility to fabricate circular cross-section channels with enlarged access holes for an easy connection with an external fluidic circuit. Moreover, a new fabrication procedure adopted allows the demonstration of microchannels with a square cross-section, thus guaranteeing an improved quality of the trapped cell images. Optical trapping and stretching of single red blood cells are demonstrated, thus proving the effectiveness of the proposed device as a monolithic optical stretcher. We believe that femtosecond laser micromachining represents a promising technique for the development of multifunctional integrated biophotonic devices that can be easily coupled to a microscope platform, thus enabling a complete characterization of the cells under test.

Paper Details

Date Published: 1 March 2010
PDF: 6 pages
Proc. SPIE 7589, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications X, 75890D (1 March 2010); doi: 10.1117/12.842137
Show Author Affiliations
N. Bellini, IFN-CNR, Politecnico di Milano (Italy)
F. Bragheri, Univ. degli Studi di Pavia (Italy)
K. C. Vishnubhatla, IFN-CNR, Politecnico di Milano (Italy)
L. Ferrara, Univ. degli Studi di Pavia (Italy)
P. Minzioni, Univ. degli Studi di Pavia (Italy)
G. Cerullo, IFN-CNR, Politecnico di Milano (Italy)
R. Ramponi, IFN-CNR, Politecnico di Milano (Italy)
I. Cristiani, Univ. degli Studi di Pavia (Italy)
R. Osellame, IFN-CNR, Politecnico di Milano (Italy)

Published in SPIE Proceedings Vol. 7589:
Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications X
Alexander Heisterkamp; Joseph Neev; Stefan Nolte; Rick P. Trebino, Editor(s)

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