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Proceedings Paper

Arbitrary-scan imaging for two-photon microscopy
Author(s): Edward Botcherby; Christopher Smith; Martin Booth; Rimas Juskaitis; Tony Wilson
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Paper Abstract

In this paper, we present details of a scanning two-photon fluorescence microscope we have built with a nearisotropic scan rate. This means that the focal spot can be scanned at high speed along any direction in the specimen, without introducing systematic aberrations. We present experimental point spread function measurements for this system using an Olympus 0.8 NA 40X water dipping objective lens that demonstrates an axial range of operation greater than 200 μm. We give details of a novel actuator device used to displace the focusing element and demonstrate axial scan responses up to 3.5 kHz. Finally, we present a bioscience application of this system to image dendritic processes that follow non-linear paths in three-dimensional space. The focal spot was scanned along one such process at 400 Hz with an axial range of more than 90 μm.

Paper Details

Date Published: 26 February 2010
PDF: 8 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756917 (26 February 2010); doi: 10.1117/12.842134
Show Author Affiliations
Edward Botcherby, Univ. of Oxford (United Kingdom)
Christopher Smith, Univ. of Oxford (United Kingdom)
Martin Booth, Univ. of Oxford (United Kingdom)
Rimas Juskaitis, Univ. of Oxford (United Kingdom)
Tony Wilson, Univ. of Oxford (United Kingdom)

Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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