Share Email Print
cover

Proceedings Paper

Multiparametric optimization of multispectral optoacoustic tomography for deep tissue imaging
Author(s): Jürgen Glatz; Nikolaos C. Deliolanis; Lu Ding; Adrian Taruttis; Amir Rosenthal; Ralf Schulz; Daniel Razansky; Vasilis Ntziachristos
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Over the last decade fluorescent reporter technologies (both fluorescent probes and proteins) have become a very powerful imaging tool in everyday biomedical research. Multispectral optoacoustic tomography (MSOT) is an emerging imaging technology that can resolve fluorophore concentration in small animals situated in deep tissue by multispectral acquisition and processing of optoacoustic signals. In this work, we study the optimum operating conditions of MSOT in imaging fluorescence activity in small animals. The performance of various fluorochromes / fluorescent proteins is examined and it is shown that the new infrared fluorescent protein is an order of magnitude brighter than the red ones. Finally, wavelength reduction after principle component analysis shows, that accurate unmixing and 3D reconstruction of the distribution of fluorochromes is possible only with 2 or 3 wavelengths.

Paper Details

Date Published: 24 February 2010
PDF: 5 pages
Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75642Z (24 February 2010); doi: 10.1117/12.842101
Show Author Affiliations
Jürgen Glatz, Technische Univ. München (Germany)
Helmholtz Zentrum München GmbH (Germany)
Nikolaos C. Deliolanis, Technische Univ. München (Germany)
Helmholtz Zentrum München GmbH (Germany)
Lu Ding, Technische Univ. München (Germany)
Helmholtz Zentrum München GmbH (Germany)
Adrian Taruttis, Technische Univ. München (Germany)
Helmholtz Zentrum München GmbH (Germany)
Amir Rosenthal, Technische Univ. München (Germany)
Helmholtz Zentrum München GmbH (Germany)
Ralf Schulz, Technische Univ. München (Germany)
Helmholtz Zentrum München GmbH (Germany)
Daniel Razansky, Technische Univ. München (Germany)
Helmholtz Zentrum München GmbH (Germany)
Vasilis Ntziachristos, Technische Univ. München (Germany)
Helmholtz Zentrum München GmbH (Germany)


Published in SPIE Proceedings Vol. 7564:
Photons Plus Ultrasound: Imaging and Sensing 2010
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

© SPIE. Terms of Use
Back to Top