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Proceedings Paper

Fluorescence of the bladder washout fluid following cystoscopy: a preliminary study
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Paper Abstract

During fluorescence cystoscopy, it is observed that the acquired images are sometimes blurred by a greenish background originating from the bladder washout fluid. Several fluorophores are involved in this overall liquid fluorescence, and their exact origin and relative contributions remain unknown. In this study, the bladder washout fluid is sampled at different times during fluorescence cystoscopy examinations. In total, 32 samples from 12 patients are analyzed with a spectrofluorimeter (excitation range: 350-445 nm, emission range 380-700 nm). This study shows clearly that the position of the fluorescence peaks (excitation/emission wavelengths: 450/525 nm, 405/625 nm) and shoulder (440/525 nm) is reproducible between different patients. It also suggests that an excitation at wavelengths higher than 400 nm helps to suppress this solution background fluorescence. Additionally, the pH of the solution seems to influence the position of the fluorescence peaks, and this suggests that changing the pH of the examination liquid could help in avoiding the greenish background.

Paper Details

Date Published: 2 March 2010
PDF: 6 pages
Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75481J (2 March 2010); doi: 10.1117/12.842100
Show Author Affiliations
Blaise Lovisa, ISIC, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Anna Maria Novello, ISIC, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Patrice Jichlinski, Ctr. Hospitalier Univ. Vaudois (Switzerland)
Hubert van den Bergh, ISIC, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Georges Wagnières, ISIC, Ecole Polytechnique Fédérale de Lausanne (Switzerland)

Published in SPIE Proceedings Vol. 7548:
Photonic Therapeutics and Diagnostics VI
Anita Mahadevan-Jansen; Andreas Mandelis; Brian Jet-Fei Wong M.D.; Nikiforos Kollias; Henry Hirschberg M.D.; Kenton W. Gregory M.D.; Reza S. Malek; E. Duco Jansen; Guillermo J. Tearney; Steen J. Madsen; Bernard Choi; Justus F. R. Ilgner; Haishan Zeng; Laura Marcu, Editor(s)

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