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Proceedings Paper

High-resolution imaging of a corneal incision by second- and third-harmonic generation microscopy
Author(s): L. Jay; C. Dion; A. Brocas; K. Singh; J.-C. Kieffer; I. Brunette; T. Ozaki
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Paper Abstract

We demonstrate high spatial resolution imaging of a stromal cut in the ex-vivo pig cornea, using second- and third-harmonic generation microscopy. From these images, we see in detail how the cut affects the corneal layers. In the beginning of the cut, the anterior layers, in which the blade is passing through, are disorganized, which could explain the shadows observed on the images. In the stroma, the cut can be imaged by third harmonic microscopy, probably due to the χ3 contrast. Although the current results were obtained from the healthy ex-vivo cornea, it already allows one to understand the effects of the cut on the tissue characteristics (such as scattering).

Paper Details

Date Published: 26 February 2010
PDF: 7 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75692T (26 February 2010); doi: 10.1117/12.842078
Show Author Affiliations
L. Jay, Maisonneuve-Rosemont Hospital Research Ctr. (Canada)
Univ. of Montreal (Canada)
Institut National de la Recherche Scientifique (Canada)
C. Dion, Maisonneuve-Rosemont Hospital Research Ctr. (Canada)
Institut National de la Recherche Scientifique (Canada)
A. Brocas, Lab. Laser, Plasmas et Procédés Photoniques (France)
K. Singh, Maisonneuve-Rosemont Hospital Research Ctr. (Canada)
Institut National de la Recherche Scientifique (Canada)
J.-C. Kieffer, Institut National de la Recherche Scientifique (Canada)
I. Brunette, Maisonneuve-Rosemont Hospital Research Ctr. (Canada)
Univ. de Montréal (Canada)
T. Ozaki, Institut National de la Recherche Scientifique (Canada)
Maisonneuve-Rosemont Hospital Research Ctr. (Canada)


Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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