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Proceedings Paper

Polarized Raman spectroscopy of bone tissue: watch the scattering
Author(s): Mekhala Raghavan; Nadder D. Sahar; Robert H. Wilson; Mary-Ann Mycek; Nancy Pleshko; David H. Kohn; Michael D. Morris
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Paper Abstract

Polarized Raman spectroscopy is widely used in the study of molecular composition and orientation in synthetic and natural polymer systems. Here, we describe the use of Raman spectroscopy to extract quantitative orientation information from bone tissue. Bone tissue poses special challenges to the use of polarized Raman spectroscopy for measurement of orientation distribution functions because the tissue is turbid and birefringent. Multiple scattering in turbid media depolarizes light and is potentially a source of error. Using a Raman microprobe, we show that repeating the measurements with a series of objectives of differing numerical apertures can be used to assess the contributions of sample turbidity and depth of field to the calculated orientation distribution functions. With this test, an optic can be chosen to minimize the systematic errors introduced by multiple scattering events. With adequate knowledge of the optical properties of these bone tissues, we can determine if elastic light scattering affects the polarized Raman measurements.

Paper Details

Date Published: 2 March 2010
PDF: 5 pages
Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 754848 (2 March 2010); doi: 10.1117/12.841977
Show Author Affiliations
Mekhala Raghavan, Univ. of Michigan (United States)
Nadder D. Sahar, Univ. of Michigan (United States)
Robert H. Wilson, Univ. of Michigan (United States)
Mary-Ann Mycek, Univ. of Michigan (United States)
Comprehensive Cancer Ctr., Univ. of Michigan (United States)
Nancy Pleshko, Temple Univ. (United States)
David H. Kohn, Univ. of Michigan (United States)
Michael D. Morris, Univ. of Michigan (United States)


Published in SPIE Proceedings Vol. 7548:
Photonic Therapeutics and Diagnostics VI
Anita Mahadevan-Jansen; Andreas Mandelis; Brian Jet-Fei Wong M.D.; Nikiforos Kollias; Henry Hirschberg M.D.; Kenton W. Gregory M.D.; Reza S. Malek; E. Duco Jansen; Guillermo J. Tearney; Steen J. Madsen; Bernard Choi; Justus F. R. Ilgner; Haishan Zeng; Laura Marcu, Editor(s)

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