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Proceedings Paper

In vivo dual-modality imaging of lymphatic systems using indocyanine green in rats: three-dimensional photoacoustic imaging and planar fluorescence imaging
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Paper Abstract

The purpose of this study is to map non-invasively sentinel lymph nodes (SLNs) and lymphatic vessels of rats in vivo using FDA-approved indocyanine green (ICG) and two non-ionizing imaging modalities: volumetric spectroscopic photoacoustic (PA) imaging, which measures optical absorption, and planar fluorescence imaging, which measures fluorescent emission. SLNs and lymphatic vessels were clearly visible after a 0.2 ml-intradermal-injection of 1 mM ICG in both imaging systems. We also imaged deeply positioned lymph nodes in vivo by layering biological tissues on top of rats. These two modalities, when used together with ICG, have the potential to map SLNs in axillary staging and to study tumor metastasis in breast cancer patients.

Paper Details

Date Published: 23 February 2010
PDF: 5 pages
Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75642W (23 February 2010); doi: 10.1117/12.841964
Show Author Affiliations
Chulhong Kim, Washington Univ. in St. Louis (United States)
Kwang Hyun Song, Univ. of Texas Southwestern Medical Ctr. at Dallas (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)

Published in SPIE Proceedings Vol. 7564:
Photons Plus Ultrasound: Imaging and Sensing 2010
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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