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Proceedings Paper

Multicolor digital holographic microscope (DHM) for biological purposes
Author(s): Zoltán Göröcs; Márton Kiss; Veronika Tóth; László Orzó; Szabolcs Tokés
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Paper Abstract

Our digital holographic (DH) approach can be used to study tissue structures both in vitro and in vivo. This DHM architecture can produce three color microscopic 3D and 4D (video) images. We record 3 color (RGB) holograms with single exposures, and the perfect compensation of color crosstalk is solved. An in-line holographic setup and reconstruction algorithms are presented with demonstrative simulations and experimentally captured and numerically reconstructed images. Comparing the individually reconstructed color images with each other can provide information both for recognition of different types of cells or microorganisms, and for diagnostic purposes as well. Experimental example is given observing microscopic hydro-biological organisms using a color digital holographic microscope.

Paper Details

Date Published: 24 February 2010
PDF: 10 pages
Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 75681P (24 February 2010); doi: 10.1117/12.841962
Show Author Affiliations
Zoltán Göröcs, Computer and Automation Research Institute (Hungary)
Márton Kiss, Computer and Automation Research Institute (Hungary)
Veronika Tóth, Computer and Automation Research Institute (Hungary)
László Orzó, Computer and Automation Research Institute (Hungary)
Szabolcs Tokés, Computer and Automation Research Institute (Hungary)


Published in SPIE Proceedings Vol. 7568:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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