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Proceedings Paper

Photoacoustic microscopy with submicron resolution
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Paper Abstract

We show that it is possible to obtain high optical contrast photoacoustic images of tissue with 0.55 μm transverse resolution. To achieve high sensitivity, we used a high NA (0.85), 125 MHz spherically focused ultrasonic transducer in a confocal arrangement with a high resolution optical objective (NA=0.6). Laser pulses of a few nJ in pulse energy with durations of 1.5 ns at a 20 KHz pulse repetition rate were used to generate photoacoustic waves. Although the penetration depth is limited to hundreds of microns by both optical scattering and ultrasonic absorption, the developed technique can compete with optical microscopy, for example, in quantitative spectral measurements, in microcirculation research, or in nanoparticle detection.

Paper Details

Date Published: 24 February 2010
PDF: 6 pages
Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75640W (24 February 2010); doi: 10.1117/12.841919
Show Author Affiliations
Konstantin Maslov, Washington Univ. in St. Louis (United States)
Geng Ku, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)

Published in SPIE Proceedings Vol. 7564:
Photons Plus Ultrasound: Imaging and Sensing 2010
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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