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Proceedings Paper

High-throughput microfluidics and ultrafast optics for in vivo compound/genetic discoveries
Author(s): Christopher B. Rohde; Cody Gilleland; Chrysanthi Samara; M. Fatih Yanik
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Paper Abstract

Therapeutic treatment of spinal cord injuries, brain trauma, stroke, and neurodegenerative diseases will greatly benefit from the discovery of compounds that enhance neuronal regeneration following injury. We previously demonstrated the use of femtosecond laser microsurgery to induce precise and reproducible neural injury in C. elegans, and have developed microfluidic on-chip technologies that allow automated and rapid manipulation, orientation, and non-invasive immobilization of animals for sub-cellular resolution two-photon imaging and femtosecond-laser nanosurgery. These technologies include microfluidic whole-animal sorters, as well as integrated chips containing multiple addressable incubation chambers for exposure of individual animals to compounds and sub-cellular time-lapse imaging of hundreds of animals on a single chip. Our technologies can be used for a variety of highly sophisticated in vivo high-throughput compound and genetic screens, and we performed the first in vivo screen in C. elegans for compounds enhancing neuronal regrowth following femtosecond microsurgery. The compounds identified interact with a wide variety of cellular targets, such as cytoskeletal components, vesicle trafficking, and protein kinases that enhance neuronal regeneration.

Paper Details

Date Published: 26 February 2010
PDF: 6 pages
Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 75680N (26 February 2010); doi: 10.1117/12.841748
Show Author Affiliations
Christopher B. Rohde, Massachusetts Institute of Technology (United States)
Cody Gilleland, Massachusetts Institute of Technology (United States)
Chrysanthi Samara, Massachusetts Institute of Technology (United States)
M. Fatih Yanik, Massachusetts Institute of Technology (United States)

Published in SPIE Proceedings Vol. 7568:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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