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Proceedings Paper

Confocal fluorescence detection for 3D cultured mammalian cells in a microfluidic cell culture system
Author(s): Jong-ryul Choi; Jong Hwan Sung; Michael L. Shuler; Donghyun Kim
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Paper Abstract

We have investigated a confocal fluorescence detection system for 3D cultured mammalian cells in a microfluidic cell culture analog system. For direct measurement of metabolic changes in the microfluidic cell culture systems using 3D cell cultures, we constructed a compact and portable confocal fluorescence detection system based on discrete optical components. The confocal detection system was designed to provide a depth scan in situ without lateral imaging for fast scanning and acquisition of average fluorescence variation associated with a large number of cells in 3D cultures of microfluidic devices. The results were consistent with the data obtained by a standard confocal microscope and suggest the potential for monitoring cell dynamics in 3D cell-based assays.

Paper Details

Date Published: 25 February 2010
PDF: 5 pages
Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 75680X (25 February 2010); doi: 10.1117/12.841729
Show Author Affiliations
Jong-ryul Choi, Yonsei Univ. (Korea, Republic of)
Jong Hwan Sung, Cornell Univ. (United States)
Michael L. Shuler, Cornell Univ. (United States)
Donghyun Kim, Yonsei Univ. (Korea, Republic of)


Published in SPIE Proceedings Vol. 7568:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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