Share Email Print
cover

Proceedings Paper

Tomographic imaging system using digital holographic technique based on integrating four buckets phase shifting interferometry
Author(s): Gihyeon Min; Ju Wan Kim; Woo Jun Choi; Eun Seo Choi; Byeong Ha Lee
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

We present a tomographic imaging system that is applied a continuous phase shifting interferometry scheme to digital holographic microscopy (DHM). The proposed scheme achieves en-face tomographic image from digitally recorded original hologram by 2-D sensor array. Although images obtained at out-of-focus position, the application of integrating four bucket technique to digital hologram produces refocused en-face image with showing clear field of view. The proposed technique has advantages such as reduced phase errors and faster acquisition speed when it compared with conventional discrete phase stepping method. The performance of the system is demonstrated with presenting of the images on a scratched mirror surface and of an USAF resolution target. The reconstructed images are compared with conventional microscopic images, which reveal good aggrements. We believe that the proposed method enables tomographic imaging of biological samples with providing reduced noise level and improved imaging speed.

Paper Details

Date Published: 26 February 2010
PDF: 6 pages
Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 756824 (26 February 2010); doi: 10.1117/12.841683
Show Author Affiliations
Gihyeon Min, Gwangju Institute of Science of Technology (Korea, Republic of)
Ju Wan Kim, Gwangju Institute of Science of Technology (Korea, Republic of)
Woo Jun Choi, Gwangju Institute of Science of Technology (Korea, Republic of)
Eun Seo Choi, Chosun Univ. (Korea, Republic of)
Byeong Ha Lee, Gwangju Institute of Science of Technology (Korea, Republic of)


Published in SPIE Proceedings Vol. 7568:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

© SPIE. Terms of Use
Back to Top