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Proceedings Paper

Low-cost two-photon microscope with fully customized trajectories
Author(s): Stefano Lodo; Alessandra Tomaselli; Carla Vacchi; Elena Ugolotti
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Paper Abstract

A modular and efficient nonlinear scanning microscope using an ultrashort pulse laser has been developed. The system is fully supervised by with an ad-hoc electronic system based on FPGA (Field Programmable Gate Array). A closed-loop control allows the compensation of scanning system non-idealities. Fully customizable trajectories can be used, in order to find best performances of the mechanical system. The electronic system is also characterized by the management of the target focusing on different focal planes and automatic research of the best focal plane. An ad-hoc software controls the system by a standard USB interface and processes images.

Paper Details

Date Published: 25 February 2010
PDF: 8 pages
Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 757016 (25 February 2010); doi: 10.1117/12.841631
Show Author Affiliations
Stefano Lodo, Univ. degli Studi di Pavia (Italy)
Alessandra Tomaselli, Univ. degli Studi di Pavia (Italy)
Carla Vacchi, Univ. degli Studi di Pavia (Italy)
Elena Ugolotti, Univ. degli Studi di Pavia (Italy)


Published in SPIE Proceedings Vol. 7570:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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