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Proceedings Paper

Applying optical Fourier filtering to standard optical projection tomography
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Paper Abstract

Light microscopy is one of the major tools in modern biology. The steady development of new microscopic techniques leads to an correspondent improvement of biological methods. To expand the catalog of biological experiments, we investigate the possibilities of optical projection tomography (OPT). This technique is based on the already established X-Ray computed tomography. In contrast to most other three-dimensional microscopy techniques it is able to create three dimensional data sets of the specimens natural absorption, staining and fluorescence. Unfortunately, these advantages are opposed by a low resolution, reconstruction artifacts, and a relatively big loss of fluorescence light. We reduced the disadvantage in resolution by applying physical filters in the Fourier plane of the image path, which is not possible in X-Ray imaging yet.

Paper Details

Date Published: 24 February 2010
PDF: 10 pages
Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 75700F (24 February 2010); doi: 10.1117/12.841599
Show Author Affiliations
Raoul-Amadeus Lorbeer, Laser Zentrum Hannover e.V. (Germany)
Heiko Meyer, Laser Zentrum Hannover e.V. (Germany)
Marko Heidrich, Laser Zentrum Hannover e.V. (Germany)
Holger Lubatschowski, Laser Zentrum Hannover e.V. (Germany)
Alexander Heisterkamp, Laser Zentrum Hannover e.V. (Germany)


Published in SPIE Proceedings Vol. 7570:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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