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Proceedings Paper

Protein kinase C delta promotes cell apoptosis induced by high fluence low-power laser irradiation
Author(s): Heng Zhang; Da Xing; Shengnan Wu; Xuegang Sun
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Paper Abstract

Protein kinase C delta (PKCδ) mediates apoptosis downstream of many apoptotic stimuli. However, whether PKCδ is involved in high fluence low-power laser irradiation (HF-LPLI)-induced cell apoptosis is still not understood. In this study, we investigated the activity of PKCδ in response to HF-LPLI treatment in human breast adenocarcinoma cells (MCF-7). Cells were transiently transfected pGFP-PKCδ and irradiated with a He-Ne laser for 10 minutes under a fluence of 120 J/cm2 after 36 hours expression. Nuclear staining with Hoechst 33258 and analysis of flow cytometry based on Annexin V-FITC/PI revealed that overexpression of PKCδ promoted the apoptotic effect of HF-LPLI. Taken together, we demonstrated that PKCδ promoted cell apoptosis caused by HF-LPLI, providing a new insight of mechanisms of HF-LPLI.

Paper Details

Date Published: 28 October 2009
PDF: 7 pages
Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 751919 (28 October 2009); doi: 10.1117/12.841566
Show Author Affiliations
Heng Zhang, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Shengnan Wu, South China Normal Univ. (China)
Xuegang Sun, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 7519:
Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009)
Qingming Luo; Lihong V. Wang; Valery V. Tuchin; Pengcheng Li; Ling Fu, Editor(s)

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