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Proceedings Paper

In vivo photodynamic inactivation of Psuedomonas aeruginosa in burned skin in rats
Author(s): Akihiro Hirao; Shunichi Sato; Mitsuhiro Terakawa; Daizoh Saitoh; Nariyoshi Shinomiya; Hiroshi Ashida; Minoru Obara
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Paper Abstract

Control of infection in wounds is critically important to avoid transition to sepsis; however, recent rise of drug-resistant bacteria makes it difficult. Thus, antimicrobial photodynamic therapy (APDT) has recently received considerable attention. In this study, we examined methylene blue (MB)-mediated photodynamic inactivation of Psuedomonas aeruginosa in rat burned skin. Two days after infection, the wound surface was contacted with a MB solution at different concentrations, and thereafter the wound was irradiated with cw 665-nm light at a constant power density of 250 mW/cm2 for different time durations. We obtained a two orders of magnitude decrease in the number of bacteria by PDT with a 2-h contact of 0.5-mM MB solution and a illumination of 480 J/cm2, demonstrating the efficacy of PDT against infection with Ps. aeruginosa in burns.

Paper Details

Date Published: 5 March 2010
PDF: 9 pages
Proc. SPIE 7551, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIX, 75510V (5 March 2010); doi: 10.1117/12.841495
Show Author Affiliations
Akihiro Hirao, Keio Univ. (Japan)
Shunichi Sato, National Defense Medical College Research Institute (Japan)
Mitsuhiro Terakawa, Keio Univ. (Japan)
Daizoh Saitoh, National Defense Medical College Research Institute (Japan)
Nariyoshi Shinomiya, National Defense Medical College (Japan)
Hiroshi Ashida, National Defense Medical College Research Institute (Japan)
Minoru Obara, Keio Univ. (Japan)


Published in SPIE Proceedings Vol. 7551:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIX
David H. Kessel, Editor(s)

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