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Proceedings Paper

Gigahertz optoacoustic imaging for cellular imaging
Author(s): Min Rui; Sankar Narashimhan; Wolfgang Bost; Frank Stracke; Eike Weiss; Robert Lemor; Michael C. Kolios
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Paper Abstract

Photoacoustic imaging exploits contrast mechanisms that depend on optical and thermomechanical properties of optical absorbers. The photoacoustic signal bandwidth is dictated by the absorber size and the laser pulse width. In this work we demonstrate that photoacoustic signals can be detected from micron and sub-micron particles. We anticipate applications to include cellular imaging with nanometer sized contrast agents such as gold nanoshells, nanorods, and nanocages. An existing acoustic microscopy system was used (the SASAM 1000, kibero GmbH). This platform is developed on an Olympus IX81 optical microscope with a rotating column that has an optical condenser for transmission optical microscopy and an acoustic module for the acoustic microscopy. The adapted optoacoustic module consists of a Qswitched Nd:YAG solid-state-laser (Teem Photonics, France) generating sub-nanosecond pulses. Scans were acquired of microparticles (1 μm black Toner particles) and cells. The confocal arrangement allowed high signal to noise ratio photoacoustic signals (>30 dB) to be detected at approximately 400 MHz. The particles of various sizes produced signals of different frequency content. In imaging mode, the full width half maximum (FWHM) was measured to be 3.6 μm for the 400 MHz transducer which is in general agreement theory for a 0.3 NA objective (4.3μm). Moreover, images are generated from single melanoma cells, generated by the endogenous contrast from the intracellular melanin.

Paper Details

Date Published: 25 February 2010
PDF: 6 pages
Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 756411 (25 February 2010); doi: 10.1117/12.841479
Show Author Affiliations
Min Rui, Ryerson Univ. (Canada)
Sankar Narashimhan, Ryerson Univ. (Canada)
Wolfgang Bost, Fraunhofer-Institut für Biomedizinische Technik (Germany)
Frank Stracke, Fraunhofer-Institut für Biomedizinische Technik (Germany)
Eike Weiss, Kibero GmbH (Germany)
Robert Lemor, Fraunhofer-Institut für Biomedizinische Technik (Germany)
Michael C. Kolios, Ryerson Univ. (Canada)

Published in SPIE Proceedings Vol. 7564:
Photons Plus Ultrasound: Imaging and Sensing 2010
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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