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Proceedings Paper

Photoacoustic detection of hemozoin in human mononuclear cells as an early indicator of malaria infection
Author(s): Jonathan R. Custer; Michael Kariuki; Brenda T. Beerntsen; John A. Viator
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Paper Abstract

Malaria is a blood borne infection affecting hundreds of millions of people worldwide2. The parasites reproduce within the blood cells, eventually causing their death and lysis. This process releases the parasites into the blood, continuing the cycle of infection. Usually, malaria is diagnosed only after a patient presents symptoms, including high fever, nausea, and, in advanced cases, coma and death. While invading the bloodstream of a host, malaria parasites convert hemoglobin into an insoluble crystal, known as hemozoin. These crystals, approximately several hundred nanometers in size, are contained within red blood cells and white blood cells that ingest free hemozoin in the blood. Thus, infected red blood cells and white blood cells contain a unique optical absorber that can be detected in blood samples using static photoacoustic detection methods. We separated the white blood cells from malaria infected blood and tested it in a photoacoustic set up using a tunable laser system consisting of an optical parametric oscillator pumped by an Nd:YAG laser with pulse duration of 5 ns. Our threshold of detection was 10 infected white blood cells per microliter, which is more sensitive than current diagnosis methods using microscopic analysis of blood.

Paper Details

Date Published: 23 February 2010
PDF: 8 pages
Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75641F (23 February 2010); doi: 10.1117/12.841399
Show Author Affiliations
Jonathan R. Custer, Univ. of Missouri, Columbia (United States)
Michael Kariuki, Univ. of Missouri, Columbia (United States)
Brenda T. Beerntsen, Univ. of Missouri, Columbia (United States)
John A. Viator, Univ. of Missouri, Columbia (United States)

Published in SPIE Proceedings Vol. 7564:
Photons Plus Ultrasound: Imaging and Sensing 2010
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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