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Proceedings Paper

Study on the best method of SPIO labeling on the cell line ECV304
Author(s): FuYuan Yang; MingXi Yu; WenLi Chen; Quan Zhou
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Paper Abstract

Studies the superparamagnetic iron oxide (SPIO) label ECV304 method and test its effect on cell physiological activity .We use different concentration PLL-SPIO incubate ECV304 cells, Prussian blue dye was taken to examine SPIO mark efficiency, DCF dyeing and PI & Annexin the VFITC double dyeing was carried out to analyze cell apoptosis. The result shows that 25μg/ml gave the most efficient labeling concentration, there is also no toxic effect on cells in this concentration. This outcome can be used in MRI of living body cell transplant tracking.

Paper Details

Date Published: 11 February 2010
PDF: 6 pages
Proc. SPIE 7565, Biophotonics and Immune Responses V, 75650P (11 February 2010); doi: 10.1117/12.841389
Show Author Affiliations
FuYuan Yang, South China Normal Univ. (China)
MingXi Yu, South China Normal Univ. (China)
WenLi Chen, South China Normal Univ. (China)
Quan Zhou, Jinan Univ. (China)


Published in SPIE Proceedings Vol. 7565:
Biophotonics and Immune Responses V
Wei R. Chen, Editor(s)

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