Share Email Print
cover

Proceedings Paper

Polarization second harmonic generation (PSHG) imaging of neurons: estimating the effective orientation of the SHG source in axons
Author(s): Sotiris Psilodimitrakopoulos; Valerie Petegnief; Guadalupe Soria; Ivan Amat-Roldan; David Artigas; Anna M. Planas; Pablo Loza-Alvarez
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We use high resolution polarization second harmonic generation (PSHG) imaging microscopy in cultured neurons, and we provide estimation on the effective orientation of the SHG source in neuronal processes in vitro. We performed pixel by pixel analysis and we found a picked distribution of angles with maximum frequency at θe = 34.54°, with Δθe=11.44°. This angle value is very close to the inclination of the tubulin heterodimmer with respect to the long axis of the microtubule.

Paper Details

Date Published: 26 February 2010
PDF: 8 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75692W (26 February 2010); doi: 10.1117/12.841313
Show Author Affiliations
Sotiris Psilodimitrakopoulos, Institut de Cièncias Fotóniques (Spain)
Valerie Petegnief, Institut de Recerca Biomèdica de Barcelona (Spain)
Guadalupe Soria, Institut de Recerca Biomèdica de Barcelona (Spain)
Ivan Amat-Roldan, Institut de Cièncias Fotóniques (Spain)
David Artigas, Institut de Cièncias Fotóniques (Spain)
Univ. Politècnica de Catalunya (Spain)
Anna M. Planas, Institut de Recerca Biomèdica de Barcelona (Spain)
Pablo Loza-Alvarez, Institut de Cièncias Fotóniques (Spain)


Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

© SPIE. Terms of Use
Back to Top