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Proceedings Paper

Fabricating multifunctional microbubbles and nanobubbles for concurrent ultrasound and photoacoustic imaging
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Paper Abstract

Background: Clinical ultrasound (US) uses ultrasonic scattering contrast to characterize subcutaneous anatomic structures. Photoacoustic (PA) imaging detects the functional properties of thick biological tissue with high optical contrast. In the case of image-guided cancer ablation therapy, simultaneous US and PA imaging can be useful for intraoperative assessment of tumor boundaries and ablation margins. In this regard, accurate co-registration between imaging modalities and high sensitivity to cancer cells are important. Methods: We synthesized poly-lactic-co-glycolic acid (PLGA) microbubbles (MBs) and nanobubbles (NBs) encapsulating India ink or indocyanine green (ICG). Multiple tumor simulators were fabricated by entrapping ink MBs or NBs at various concentrations in gelatin phantoms for simultaneous US and PA imaging. MBs and NBs were also conjugated with CC49 antibody to target TAG-72, a human glycoprotein complex expressed in many epithelial-derived cancers. Results: Accurate co-registration and intensity correlation were observed in US and PA images of MB and NB tumor simulators. MBs and NBs conjugating with CC49 effectively bound with over-expressed TAG-72 in LS174T colon cancer cell cultures. ICG was also encapsulated in MBs and NBs for the potential to integrate US, PA, and fluorescence imaging. Conclusions: Multifunctional MBs and NBs can be potentially used as a general contrast agent for multimodal intraoperative imaging of tumor boundaries and therapeutic margins.

Paper Details

Date Published: 25 February 2010
PDF: 5 pages
Proc. SPIE 7567, Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue II, 75670L (25 February 2010); doi: 10.1117/12.841281
Show Author Affiliations
Ruogu Qin, The Ohio State Univ. (United States)
Jeff Xu, The Ohio State Univ. (United States)
Ronald Xu, The Ohio State Univ. (United States)
Chulhong Kim, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)


Published in SPIE Proceedings Vol. 7567:
Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue II
Robert J. Nordstrom, Editor(s)

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