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Proceedings Paper

Effect of low level laser therapy on hair cell regeneration following gentamicin induced ototoxicity in postnatal organotypic culture of rat cochlea
Author(s): Chung-Ku Rhee; Young Hoon Kim; Se Hyung Kim; Peijie He; Jin Chul Ahn
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Paper Abstract

Aim: To investigate effects of low level laser therapy (LLLT) on hair cell regeneration following gentamicin ototoxicity in organotypic culture of rat cochlea. Methods: Organotypic cultures of cochlea in culture medium were allowed to grow for 17 days (C group). The organotypic cultures were irradiated daily with 808 nm LD laser, at 28.8 J/ cm2(L group). The organotypic culture were exposed to 1 mM of gentamicin for 48 hr and allowed to recover (G group) or allowed to recover in the culture medium with daily LLLT at 28.8 J/ cm2 (GL group) for 17 days. The cochleae were stained with FM1-43. The number of hair cells was counted in each group serially for 17 days. Results: While the C group kept on losing hair cells in vitro culture, the hair cells remained rather stationary in the L group. The number of hair cells revealed significantly larger number of hair cells in the L group compared to the C group (p=0.05). And the group × time interaction was also significant (p=0.04). That is, the number of hair cells in the C group showed decreasing tendency which was significantly different from the L group. In G group, the initial number of hair cells decreased to 37.2% of that of the gentamicin non-exposed groups. While the G group kept on losing hair cells, the number of hair cells increased in the GL group. The number of hair cells revealed significantly larger in the GL group (p=0.01) compared to G group. And the group × time interaction was also significant (p=0.01). Also, the number of hair cells in the GL group showed increasing tendency which was significantly different from the G group. Conclusion: These results suggest that LLLT promotes hair cell regeneration following gentamicin damage in cochlear explants.

Paper Details

Date Published: 25 February 2010
PDF: 7 pages
Proc. SPIE 7552, Mechanisms for Low-Light Therapy V, 75520B (25 February 2010); doi: 10.1117/12.841267
Show Author Affiliations
Chung-Ku Rhee, Dankook Univ. College of Medicine (Korea, Republic of)
Young Hoon Kim, Dankook Univ. College of Medicine (Korea, Republic of)
Se Hyung Kim, Dankook Univ. College of Medicine (Korea, Republic of)
Peijie He, Dankook Univ. College of Medicine (Korea, Republic of)
Jin Chul Ahn, Dankook Univ. College of Medicine (Korea, Republic of)


Published in SPIE Proceedings Vol. 7552:
Mechanisms for Low-Light Therapy V
Michael R. Hamblin; Ronald W. Waynant; Juanita Anders, Editor(s)

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