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Proceedings Paper

Use of a cyanine dye probe to estimate the composition of the vitreous body after enzymatic treatment
Author(s): Ina G. Panova; Alexander S. Tatikolov; Natalia P. Sharova
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Paper Abstract

The aim of this work was to study the effect of enzymes such as proteinase K, trypsin, collagenase with hyaluronidase, as well as a mixture of all these enzymes, on albumin and collagens incorporated in the vitreous body, using a cyanine dye as a spectral-fluorescent probe. We studied the vitreous body of the eyes of 19/20-week human fetuses, in which, as we showed earlier, the concentration of albumin in the vitreous body is sufficiently high. Proteinase K steeply decreased the albumin content in the vitreous body, whereas trypsin and hyaluronidase with collagenase had no effect on the albumin content. Collagen was not subjected to proteinase K. Enzymatic digestion of collagen occurred under the action of collagenase with hyaluronidase. The content of albumin and collagen sharply decreased in the system after treatment of the vitreous body with mixture of all enzymes. Hence, the results obtained showed that, even being in the mixture, these enzymes have a selective effect on albumin and collagens. The possibility to study the dose-dependent character of enzymatic vitreolysis using a cyanine dye probe has been shown. The spectral-fluorescent probe for albumin and collagens proved to be useful for experimental approaches at screening the enzymatic mixtures possessing the selective action. The study performed is considered as a preclinical trial, and the method presented as promising for the further research in this field. The effect of the enzymes used for therapeutic purposes on the functional conditions of the vitreous body should be studied.

Paper Details

Date Published: 2 March 2010
PDF: 7 pages
Proc. SPIE 7550, Ophthalmic Technologies XX, 75501W (2 March 2010); doi: 10.1117/12.841128
Show Author Affiliations
Ina G. Panova, Institute of Developmental Biology (Russian Federation)
Alexander S. Tatikolov, Institute of Biochemical Physics (Russian Federation)
Natalia P. Sharova, Institute of Developmental Biology (Russian Federation)


Published in SPIE Proceedings Vol. 7550:
Ophthalmic Technologies XX
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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