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Proceedings Paper

Comparison of cellular responses induced by low level light in different cell types
Author(s): Ying-Ying Huang; Aaron C.-H. Chen; Sulbha K. Sharma; Qiuhe Wu; Michael R. Hamblin
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Paper Abstract

Discoveries are rapidly being made in multiple laboratories that shed "light" on the fundamental molecular and cellular mechanisms underlying the use of low level light therapy (LLLT) in vitro, in animal models and in clinical practice. Increases in cellular levels of respiration, in cytochrome c oxidase activity, in ATP levels and in cyclic AMP have been found. Increased expression of reactive oxygen species and release of nitric oxide have also been shown. In order for these molecular changes to have a major effect on cell behavior, it is likely that various transcription factors will be activated, possibly via different signal transduction pathways. In this report we compare and contrast the effects of LLLT in vitro on murine embryonic fibroblasts, primary cortical neurons, cardiomyocytes and bone-marrow derived dendritic cells. We also examined two human cell lines, HeLa cancer cells and HaCaT keratinocytes. The effects of 810-nm near-infra-red light delivered at low and high fluences were addressed. Reactive oxygen species generation, transcription factor activation and ATP increases are reported. The data has led to the hypothesis that cells with a high level of mitochondrial activity (mitochondrial membrane potential) have a higher response to light than cells with low mitochondrial activity.

Paper Details

Date Published: 26 February 2010
PDF: 10 pages
Proc. SPIE 7552, Mechanisms for Low-Light Therapy V, 75520A (26 February 2010); doi: 10.1117/12.841018
Show Author Affiliations
Ying-Ying Huang, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Guangxi Medical Univ. (China)
Aaron C.-H. Chen, Massachusetts General Hospital (United States)
Boston Univ. School of Medicine (United States)
Sulbha K. Sharma, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Qiuhe Wu, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Jinan Central Hospital, Shandong Univ. (China)
Michael R. Hamblin, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Harvard-MIT Div. of Health Sciences and Technology (United States)


Published in SPIE Proceedings Vol. 7552:
Mechanisms for Low-Light Therapy V
Michael R. Hamblin; Ronald W. Waynant; Juanita Anders, Editor(s)

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