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Proceedings Paper

Label-free classification of cell types by imaging of cell membrane fluctuations using low-coherent full-field quantitative phase microscopy
Author(s): Toyohiko Yamauchi; Norikazu Sugiyama; Takashi Sakurai; Hidenao Iwai; Yutaka Yamashita
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Paper Abstract

Cell membrane motions of living cells are quantitatively measured in nanometer resolution by low-coherent full-field quantitative phase microscopy. Our setup is based on a full-field phase shifting interference microscope with a very lowcoherent light source. The reflection mode configuration and the low-coherent illumination make it possible to differentiate the weak reflection light from the cell membrane from the strong reflection from the glass substrate. Two cell populations are quantitatively assessed by the power spectral density of the cell surface motion and show different trends.

Paper Details

Date Published: 25 February 2010
PDF: 8 pages
Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 75700X (25 February 2010); doi: 10.1117/12.841008
Show Author Affiliations
Toyohiko Yamauchi, Hamamatsu Photonics K.K. (Japan)
Massachusetts Institute of Technology (United States)
Norikazu Sugiyama, Hamamatsu Photonics K.K. (Japan)
Takashi Sakurai, Hamamatsu Univ. School of Medicine (Japan)
Hidenao Iwai, Hamamatsu Photonics K.K. (Japan)
Yutaka Yamashita, Hamamatsu Photonics K.K. (Japan)


Published in SPIE Proceedings Vol. 7570:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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