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Proceedings Paper

Quantification of colloidal and intracellular gold nanomarkers down to the single particle level using confocal microscopy
Author(s): Sabine Klein; Svea Petersen; Ulrike Taylor; Detlef Rath; Stephan Barcikowski
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Paper Abstract

The high quantum yield and exclusively photo-stable excitation of gold nanoparticles combined with their bio-inert characteristics make them ideal cellular markers. The aim of the study was to visualise gold nanoparticles size-dependently as colloid and in cells after co-incubation. We show the quantification of colloidal gold particles by standard confocal microscopy down to the single particle level. A calibration is demonstrated for pixel numbers in dilution series of uncoated gold nanoparticles. We give implications for practical use of advanced cellular imaging in cultured cells.

Paper Details

Date Published: 26 February 2010
PDF: 6 pages
Proc. SPIE 7573, Biomedical Applications of Light Scattering IV, 75730L (26 February 2010); doi: 10.1117/12.840985
Show Author Affiliations
Sabine Klein, Friedrich-Loeffler-Institut (Germany)
Svea Petersen, Laser Zentrum Hannover e.V. (Germany)
Excellence Cluster Rebirth (Germany)
Ulrike Taylor, Friedrich-Loeffler-Institut (Germany)
Detlef Rath, Friedrich-Loeffler-Institut (Germany)
Stephan Barcikowski, Laser Zentrum Hannover e.V. (Germany)
Excellence Cluster Rebirth (Germany)


Published in SPIE Proceedings Vol. 7573:
Biomedical Applications of Light Scattering IV
Adam P. Wax; Vadim Backman, Editor(s)

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