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Proceedings Paper

Investigation of pore structure and cell distribution in EH-PEG hydrogel scaffold using optical coherence tomography and fluorescence microscopy
Author(s): Chao-Wei Chen; Martha W. Betz; John P. Fisher; Andrew Paek; James Jiang; Hongzhou Ma; Alex Cable; Yu Chen
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Paper Abstract

Macroporous EH-PEG hydrogels fabricated by porogen-leaching method are characterized by optical coherence tomography (OCT). High-resolution OCT visualizes the microstructures of the engineered tissue scaffolds in threedimensions. It also enables subsequent image processing to investigate several key morphological design parameters for macroporous scaffolds. Image processing algorithms are then presented to automatically quantify the pore size, porosity, and pore interconnectivity. The results indicated that those parameters highly depend on the porogen size. Further, fluorescence imaging was conducted to monitor the population of labeled human mesenchymal stem cells (hMSCs) loaded on the surface of the scaffolds. The results revealed the hMSCs' viability as well as their infiltration into the scaffold. The effect of infiltration is more profound in the scaffold of larger pore sizes, in accordance with the result suggested by image analysis.

Paper Details

Date Published: 11 February 2010
PDF: 6 pages
Proc. SPIE 7566, Optics in Tissue Engineering and Regenerative Medicine IV, 756603 (11 February 2010); doi: 10.1117/12.840809
Show Author Affiliations
Chao-Wei Chen, Univ. of Maryland, College Park (United States)
Martha W. Betz, Univ. of Maryland, College Park (United States)
John P. Fisher, Univ. of Maryland, College Park (United States)
Andrew Paek, Univ. of Maryland, College Park (United States)
James Jiang, Thorlabs, Inc. (United States)
Hongzhou Ma, Thorlabs, Inc. (United States)
Alex Cable, Thorlabs, Inc. (United States)
Yu Chen, Univ. of Maryland, College Park (United States)


Published in SPIE Proceedings Vol. 7566:
Optics in Tissue Engineering and Regenerative Medicine IV
Sean J. Kirkpatrick; Ruikang Wang, Editor(s)

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